Table 3.

Kinetic parameters (with D-glucose as electron donor and O₂ as electron acceptor) of P2Ox mutants compared to the total interaction energy between protein and 2-FG.

Parameter a	3K4L	4MOF	4MOJ
Total Eel [kJ mol−1]	−512.3	−490.0	−635.1
Michaelis constant (Km) [mM]	1.5 ± 0.1	0.939 ± 0.04	2.43 ± 0.27
Turnover number (kcat) [s−1]	12.0 ± 0.0	12.5 ± 0.2	16.8 ± 0.27
Specificity constant (Km/kcat) [mM s−1]	8.2	12.7	13.5

^a parameters were taken from the literature [44,45].