Table 1.
Target Gene | 5′-3′ Forward Primer Sequence | 5′-3′ Reverse Primer Sequence | Product Length |
---|---|---|---|
GAPDH | CAA CTC CCT CAA GAT TGT CAG CAA | GGC ATG GAC TGT GGT CAT GA | 118 |
Myf5 | CTA TTA CAG CCT GCC GGG AC | CTC GGA TGG CTC TGT AGA CG | 232 |
MyoD | TGC TCC TTT GAG ACA GCA GA | AGT AGG GAA GTG TGC GTG CT | 141 |
Myogenin | AGG AGA GAA AGA TGG AGT CCA GAG | TAA CAA AAG AAG TCA CCC CAA GAG | 430 |
MRF4 | AGG GCT CTC CTT TGT ATC CAG | TGG AAG AAA GGC GCT GAA GA | 579 |
IGF-1Ea | GTG GAC GCT CTT CAG TTC GT | GCT TCC TTT TCT TGT GTG TCG ATA G | 262 |
IGF-1Eb | GTC CCC AGC ACA CAT CGC G | TCT TTT GTG CAA AAT AAG GCG TA | 259 |
FUCA | TTT GGT CGG TGA GTT GGG AG | CCA TTC CAA GAG CGA GTG GT | 76 |
FoxO1 | AGT GGA TGG TGA AGA GCG TG | GAA GGG ACA GAT TGT GGC GA | 96 |
p53 | GAG AGA CCG CCG TAC AGA AG | AGC AGT TTG GGC TTT CCT CC | 317 |
Myostatin | CTG TAA CCT TCC CAG GAC CA | GCA GTC AAG CCC AAA GTC TC | 104 |
MuRF1 | AGG GCT CCC CAC CAC CTG TGT | TGC CCT CTC TAG GCC ACC G | 310 |
Atrogin1/
MAFbx |
AAC AAG GAG GTA TAC AGT AAG G | AAT TGT TCA TGA AGT TCT TTT G | 322 |
IL-6 | CTA TGA ACT CCT TCT CCA CAA GCG CCT T | GGG GCG GCT ACA TCT TTG GAA TCT T | 301 |
PPAR-γ | GTT CAT GCT TGT GAA GGA TGC | ACT CTG GAT TCA GCT GGT CG | 359 |