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. 2021 Apr 2;22(7):3721. doi: 10.3390/ijms22073721

Table 1.

The sequences of the specific sets of primers used for RT-PCR analyses.

Target Gene 5′-3′ Forward Primer Sequence 5′-3′ Reverse Primer Sequence Product Length
GAPDH CAA CTC CCT CAA GAT TGT CAG CAA GGC ATG GAC TGT GGT CAT GA 118
Myf5 CTA TTA CAG CCT GCC GGG AC CTC GGA TGG CTC TGT AGA CG 232
MyoD TGC TCC TTT GAG ACA GCA GA AGT AGG GAA GTG TGC GTG CT 141
Myogenin AGG AGA GAA AGA TGG AGT CCA GAG TAA CAA AAG AAG TCA CCC CAA GAG 430
MRF4 AGG GCT CTC CTT TGT ATC CAG TGG AAG AAA GGC GCT GAA GA 579
IGF-1Ea GTG GAC GCT CTT CAG TTC GT GCT TCC TTT TCT TGT GTG TCG ATA G 262
IGF-1Eb GTC CCC AGC ACA CAT CGC G TCT TTT GTG CAA AAT AAG GCG TA 259
FUCA TTT GGT CGG TGA GTT GGG AG CCA TTC CAA GAG CGA GTG GT 76
FoxO1 AGT GGA TGG TGA AGA GCG TG GAA GGG ACA GAT TGT GGC GA 96
p53 GAG AGA CCG CCG TAC AGA AG AGC AGT TTG GGC TTT CCT CC 317
Myostatin CTG TAA CCT TCC CAG GAC CA GCA GTC AAG CCC AAA GTC TC 104
MuRF1 AGG GCT CCC CAC CAC CTG TGT TGC CCT CTC TAG GCC ACC G 310
Atrogin1/
MAFbx
AAC AAG GAG GTA TAC AGT AAG G AAT TGT TCA TGA AGT TCT TTT G 322
IL-6 CTA TGA ACT CCT TCT CCA CAA GCG CCT T GGG GCG GCT ACA TCT TTG GAA TCT T 301
PPAR-γ GTT CAT GCT TGT GAA GGA TGC ACT CTG GAT TCA GCT GGT CG 359