Table 4.

Recoveries obtained in FBS by the optimized MSPE procedure followed by high-performance liquid chromatography coupled with mass spectrometry (HPLC–MS/MS) (n = 3).

		Mean Recovery (%) 1
	Spike (ng mL−1)	100	25	5	1 2
PREDLO		95	84	87	65
PRED		109	98	97	107
H-CORT		80	80	71	70
CORT		91	87	97	70
BETA		97	94	104	122
DEXA		97	96	80	75
TRIAM		100	95	110	104
E2		106	115	109 2	n.q. 3
TST		84	81	95	92
EPI		84	82	94	94
EE2		89	82	88	97
E1		86	89	105	90
H-PROG		105	90	84	96
FLUO		75	91	97	106
PROG		88	89	80	84
M-PROG		98	101	99	82

¹ RSDs < 14%, n = 3. ² evaporation of the MSPE eluate and reconstitution in 0.25 mL MeOH (EF 4) before analysis. ³ n.q., not quantifiable at this concentration level.