Table 5.
Results of robustness analysis of CCHO and EOH by changing mobile phase compositions for sustainable HPTLC technique (mean ± SD; n = 6).
| Conc. (ng band−1) |
Mobile Phase Composition (Cyclohexane/Ethyl Acetate) | Results | ||||
|---|---|---|---|---|---|---|
| Original | Used | (ng band−1) ± SD | % CV | Rf | ||
| CCHO | ||||||
| 92:8 | +2.0 | 511.23 ± 3.10 | 0.60 | 0.26 | ||
| 500 | 90:10 | 90:10 | 0.0 | 514.62 ± 3.35 | 0.65 | 0.27 |
| 88:12 | −2.0 | 516.41 ± 3.52 | 0.68 | 0.28 | ||
| EOH | ||||||
| 92:8 | +2.0 | 489.22 ± 3.70 | 0.75 | 0.37 | ||
| 500 | 90:10 | 90:10 | 0.0 | 491.80 ± 3.90 | 0.79 | 0.38 |
| 88:12 | −2.0 | 498.28 ± 4.28 | 0.85 | 0.39 | ||