Table 1.
Most recent pre-clinical studies (last 10 years) investigating the role of human lipoaspirate on BC.
| Reference | Study Setting | AT Component | BC Model | Results |
|---|---|---|---|---|
| Almarzouqi et al. [135] | In vitro | Whole AT | MCF-7 cells | Increased BC proliferation rate; triggered expression of MMP1, integrin α2 |
| Massa et al. [136] | In vitro | Whole AT; in vitro differentiated adipocytes | MDA-MB-231, MCF-7, ZR-75 cells | Increased BC proliferation |
| Rowan et al. [137] | In vitro, in vivo |
ASCs | MDA-MB-231 cells | Stimulated BC migration; stimulated tumor growth by ASCs from BMI 18.3 patient but not BMI 25.0 patient |
| Kucerova et al. [138] | In vitro | ASCs | SKBR3 cells | Increased BC migration, mammosphere formation, EMT, chemosensitivity |
| Devarajan et al. [139] | In vitro | ASCs | T47D, MCF-7, BT-474 cells | Induced EMT; promoted PDGF-D mediated colony formation |
| Lin et al. [140] | In vitro | ASCs | MCF7 cells | Promoted BC migration through ASCs-EVs |
| Eterno et al. [141] | In vitro, in vivo |
Autologous ASCs | BC cells from human donors; MDA-MB-231, MCF-7 cells | Enhanced aggressiveness in MDA-MB-231 and in 50% of patient-derived BC |
| Wu et al. [142] | In vitro | ASCs | MDA-MB-231, MDA-MB-468, MCF-7 cells | Reduced BC viability |
| Orecchioni et al. [143] | In vitro, in vivo |
Whole AT, ASCs, endothelial progenitors | MDA-MB-436, HCC1937 cells | Induced EMT; increased tumor volume and lung metastases |
| Tsuji et al. [144] | In vivo | lipograft | MDA-MB-231, BT-474 xenografts | Reduced engraftment; decreased BC proliferation |
| Silva et al. [145] | In vivo | Whole AT | MCF-7 xenograft | Reduced tumor volume and Ki67 |
| Sabol et al. [146] | In vitro, in vivo |
obASCs | TNBC cell lines; TNBC patient-derived xenograft | Promoted metastasis through leptin signaling |
| Sabol et al. [147] | In vitro, in vivo |
obASCs | MCF7-Y537S cells; PDX models WHIM20 (Y537S mutation) and WHIM43 (D538G mutation) | Promoted metastasis in BC with mutant ERα; in vitro obASCs promoted proliferation and migration of ER WT and ER MUT cells |
AT, adipose tissue; ASCs, adipose-derived stromal cells; BC, breast cancer, EMT, epithelial–mesenchymal transition; EVs, extracellular vesicles; obASCs, obesity-altered adipose stem cells; WAT, white adipose tissue.