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. 2021 Apr 3;13(7):1698. doi: 10.3390/cancers13071698

Figure 5.

Figure 5

CD4+ T cell polarization is reproductive stage dependent with implications for antigen-specific activation. (a) Flow cytometry phenotyping of CD45+ CD4+ T cells from livers of mice at nullip, InvD2, and InvD6 reproductive stages (nullip n = 8, InvD2 n = 5, InvD6 n = 6). Only CD45+ CD4+ T cells that expressed at least one of the four phenotyping markers RORgT, Tbet, PD1, or FoxP3 are shown (see Figure S3 for total CD45+CD4+ T cell polarization); (b) Experimental schema for the in vivo T cell activation assay (Figure S4 shows transferred CD4+ Do11.10 cells); Representative flow cytometry plots showing expression of ova-specific Do11.10 T cell receptor (TCR) in CD45+ CD4+ CD8- cells from mice intra-hepatically injected with (c) ovalbumin (OVA) antigen or (d) PBS; Flow cytometry quantification of ova-specific CD4+ T cells (Do11.10 TCR+) as %CD4+ in (e) whole liver or (f) spleen across 2 independent experiments (nullip PBS n = 16, nullip OVA n=15, InvD2 PBS n = 13, InvD2 OVA n = 13). Data normalized to PBS average for reproductive group, One-way ANOVA. * p < 0.05, *** p < 0.001, **** p < 0.0001.