Figure 10.

ZNF71 isoforms in paclitaxel-resistant (H460-R) and parental (H460-P) cell lines. (A) Comparison of fold change of ZNF71 overall, KRAB, and KRAB-less expression in H460-P and H460-R cell lines in qRT-PCR experiments. * p < 0.05, *** p < 0.001, NS: non-significance, two-sample t-tests. (B,C) H460-P (B) and H460-R (C) cells were treated either with DMSO or Taxol with the indicated doses. After 48 h, the cells were stained with Annexin V and PI and the percent of live/dead cells was determined by flow cytometry. Error bars represent standard deviation from three biological replicates. A mixed-effect model was used to assess the difference between two conditions, using R package Ime4. The p-value was calculated based on asymptotic Z-distribution. (D) Flow cytometry images for H460-P and H460-R, respectively, after treatment with either DMSO or Taxol.