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. 2021 Apr 3;22(7):3750. doi: 10.3390/ijms22073750

Figure 4.

Figure 4

ZIKV inhibits CHOP protein expression in the infected cells. (a) Immunofluorescence detection of ZIKV envelope (ZIKV-E, green) expressing cells and CHOP protein (red) in A549 cells infected or not with ZIKV at MOI = 5 for 24 h, further treated or not with TG for 4 h at 20 h.p.i and cells treated with TG for 4 h. Nuclei were stained with DAPI (blue). h.p.i: hours post-infection. In the microscopic field of cells infected with ZIKV and treated with TG for 4 h at 20 h.p.i, the green arrow points a cell stained for ZIKV-E without CHOP staining in the nucleus and the red arrow points a cell which is not stained for ZIKV-E but has a CHOP stain in the nucleus. (b) A549 cells were infected or not with ZIKV at MOI 5 for 24 h, treated 20 h.p.i with TG for 4 h or only treated with TG for 4 h. The cells were harvested at the different conditions indicated above and subjected to western blot analysis using antibodies against CHOP and ZIKV envelope protein (4 G2). β-Tubulin was used as a loading control.