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Effect of miR205HG-stable transfection on proliferation of EAC cells. miR205HG stably transfected clones were studied in water-soluble tetrazolium salt (WST-1) assays to detect the effect of exogenous miR205HG overexpression on proliferation of SKGT4 and FLO-1 EAC cell lines. Compared to vector-only transfected negative control, forced overexpression of miR205HG decreased cell proliferation on Day 5 by 45% and 69% in SKGT4 and FLO-1 cells, respectively (** p-values all < 0.001).
