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. 2021 Apr 3;13(7):1707. doi: 10.3390/cancers13071707

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Significant reduction in SHH protein levels in SKGT4 and FLO-1 miR205HG-stably transfected clones. Western blotting demonstrated that SHH protein levels were greatly reduced in SKGT4 and FLO-1 miR205HG-stably transfected clones. Flo (−) and SK (−) denote FLO-1 and SKGT4 cells stably transfected with empty vector only. Flo lnc1 and lnc2 represent two different FLO-1 miR205HG stably transfected clones (the same labeling applies to SKGT4 cells). (full blots can be found in Supplementary Materials).