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Significant PTCH1 downregulation induced by miR-205 mimic transfection in EAC cells. After transfecting FLO-1 EAC cells with a negative control mimic or a miR-205 mimic, qRT-PCR was performed for four genes (SHH, PTCH1, SMO, GLI1). Only PTCH1 showed a significant (** p-value = 0.0001) reduction when transfected with 60 nM of miR-205 mimic.
