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Significant reduction in luciferase activity induced by full-length 3′UTR insert in FLO-1 and AGS cells. Relative to luciferase activity after pmiRGLO vector-only transfection, the full-length 3′UTR insert caused the most significant reduction of luciferase activity in both FLO-1 and AGS cells. Vector + Frag1, 2, and 3 denote three different truncated PTCH1 3′UTR constructs.
