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HbS correction with prime editing.
Schematic representing the gene editing approach by Anzalone et al[100] used to correct the HbS mutation in HEK293 cells. This method is a direct correction of the pathogenic T:A base pair to A:T (a transversion) that does not require the use of an exogenous donor template or DSB. nCas9: Cas9 nickase; RT: reverse transcriptase; pegRNA: prime editing gRNA.
