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. 2021 Apr 5;13(7):1718. doi: 10.3390/cancers13071718

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Increased lipid unsaturation level in ovarian cancer cells represented by intensity ratio between 2900 and 3002 cm^‒1. (A) Representative hyperspectral SRS images of flow-sorted ALDH⁻/CD133⁻ and ALDH⁺/CD133⁺ COV362 cells. Images at 2900 and 3002 cm^‒1, and the intensity ratio image between 3002 and 2900 cm⁻¹ are shown. Scale bars: 10 μm. (B) Average SRS spectra from the lipid droplets in ALDH⁻/CD133⁻ (n = 3) and ALDH⁺/CD133⁺ cells (n = 8). Shaded area indicates the standard deviation of SRS spectral measurement from different cells. Reprinted and adapted with permission from reference [48].