Fig. 2 ∣. Inflammasome activation promotes macrophage proliferation and features of plaque instability in mice with Jak2^VF bone marrow.

a, Immunofluorescence images of aortic root plaques. Arrows, Ki67⁺ nuclei. Scale bar, 50 μm. b, Percentage Ki67⁺ cells in lesions. c, Macrophage number normalized to total cells in lesions (n = 8 mice (b, c)). d, Necrotic core area (n = 9 control, n = 8 Casp1/11^−/−Jak2^VF and Jak2^VFCasp1/11^−/− mice). e, Cap thickness (n = 8 mice). f, Lesion area (n = 9 control, n = 8 Casp1/11^−/−Jak2^VF and Jak2^VFCasp1/11^−/− mice). g, Immunofluorescence staining of aortic root lesions from mice with Jak2^VF clonal haematopoiesis. Arrows, pγH2AX⁺ nuclei. Scale bars, 100 μm. h, pγH2AX⁺MAC2⁺ cells normalized to lesion area (n = 19 control, n = 20 Jak2^VF mice). i, Representative immunofluorescence staining of aortic roots. Yellow dashed lines, lesion. j, 8-oxo-deoxyguanosine (8-OHdG) fluorescence intensity normalized to lesion area (n = 17 mice). k, Representative H&E images of aortic root lesions from mice with Jak2^VF or Jak2^VFNlrp3^−/− bone marrow. Yellow dashed lines, necrotic cores. l, m, Quantification of lesion area (l; n = 15 mice) and percentage necrotic core area (m; n = 19 mice). n, Representative H&E images of aortic root lesions from mice with Jak2^VF (n = 24) or Jak2^VFAim2^−/− (n = 25) bone marrow. Yellow dashed lines, necrotic core. o, p, Lesion area (o) and percentage necrotic core area (p). Mean ± s.e.m.; two-tailed t-test (l, m, o, p); Kruskal–Wallis two-tailed test with Dunn’s comparison (b, c, e); one-way ANOVA with Tukey’s post hoc analysis (d, f); two-way ANOVA (e); two-tailed Mann–Whitney test (h, j).