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. 2021 Apr 6;22(7):3776. doi: 10.3390/ijms22073776

Figure 3.

Figure 3

Antibody-inducing activity of artificially synthesized GSLs (artGSLs). (A) Chemical structures of artGSLs used for immunization experiments. The ceramide mimetics C12S and C12L are composed of a saturated C12-sphingosine mimetic and stearic acid (C18:0) or lignoceric acid (C24:0), respectively. These ceramide mimetics are bound to the oligosaccharide via a β-linkage. Abbreviations: 6SLN, 6′-sialyl LacNAc/Neu5Acα2,6Galβ1,4GlcNAc; sLeX, sialyl LewisX/Neu5Acα2,3Galβ1,4(Fucα1,3)GlcNAc; LeX, LewisX/Galβ1,4(Fucα1,3)GlcNAc; CF4, core-fucosylated tetrasaccharide/Manβ1,4GlcNAcβ1,4(Fucα1,6)GlcNAc. (B) Reactivity of mice serum IgMs against immunizing GSL. Mice were immunized with each artGSL, and serum samples were prepared 7 days after immunization, as described previously [15]. Reactivity of serum antibodies against immunizing GSLs was analyzed by ELISA (A450). Abbreviations; BG, serum from untreated mice; D7, serum prepared from mice immunized with each GSL 7 days after immunization. Diamonds indicate individual mouse serum samples (n = 8–13). Solid lines indicate average reactivity of serum samples. Values include previously reported data [13,15].