Figure 3.
Anandamide (AEA) and 2-arachidonoylglycerol (2-AG) contents, fatty acid amide hydrolase (FAAH) and monoacylglycerol lipase (MAG-L) levels in the nucleus accumbens (NAc) after mSPS/Control or Air/CIE exposures. (A) Schematic of coronal slice where NAc tissue punches were taken bilaterally (adapted from [58]). Average (B) AEA content (Control-Air: n = 3; Control CIE: n = 5; mSPS-Air: n = 6; mSPS-CIE: n = 6) and (C) FAAH levels (Control-Air: n = 7; Control CIE: n = 4; mSPS-Air: n = 5; mSPS-CIE: n = 8) (Inset: representative immunoblotting sample images) did not change among groups after mSPS/Control or Air/CIE exposures. The average (D) 2-AG content (Control-Air: n = 3; Control CIE: n = 6; mSPS-Air: n = 6; mSPS-CIE: n = 7) and the average (E) MAG-L levels (Control-Air: n = 7; Control CIE: n = 4; mSPS-Air: n = 5; mSPS-CIE: n = 8) (Inset: representative immunoblotting sample images) did not change among groups after mSPS/Control or Air/CIE exposures. Data are mean ± SEM.