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The HO-1 protein expression by ginsenoside is regulated through EGFR. The HPDL cells (5 × 104 cells/mL) were cultured for 24 h and then treated with 100 pmol of EGFR siRNA for 24 h to induce EGFR silencing. Thereafter, each ginsenoside-Re (A), -Ra8 (B), and -Rf (C) were treated at the indicated concentration for 6 h, and then PG-LPS was treated for 24 h. After, the protein amount and gene level were measured using Western blot analysis and real time PCR analysis.
