Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The HPLC conditions for quantitative analysis.
| Parameters | Conditions | ||
|---|---|---|---|
| Analytical column | Phenomenex C18 (4.6 × 250 mm) | ||
| HPLC detector | Diode array detector (203 nm) | ||
| Solvent A: Water Solvent B: ACN |
|||
| Mobile phase | Final time | Solvent | |
| (min) | A (%) | B (%) | |
| 0 | 83 | 17 | |
| 20 | 75 | 25 | |
| 38 | 58 | 42 | |
| 60 | 83 | 17 | |
| Flow rate | 0.8 mL/min | ||
| Column oven temperature | 30 °C | ||
| Injection volume | 10 μL | ||
| Run time | 60 min | ||
