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. 2021 Apr 6;22(7):3782. doi: 10.3390/ijms22073782

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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FN, vimentin (VIM), and transforming growth factor-beta (TGF-β) expressions are increased during the progression of low-grade astrocytoma into glioblastoma multiforme (GBM). Serial images including first and follow-up examinations of a patient with GBM are shown by using either brain computerized tomography (CT) on 25 October 2010 (A) and 8 December 2010 (C) or head MRI on 25 October 2010 (B), 5 May 2011 (D), 28 December 2012 (E) and 17 May 2013 (F). The IHC staining images indicate the expression level of FN (G and H), VIM (I and J), and TGF-β (K and L) proteins in the origin of low-grade astrocytoma (G, I, and K) and local recurrence of GBM (H, J, and L) tumor specimens.