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. 2021 Apr 6;22(7):3783. doi: 10.3390/ijms22073783

Figure 3.

Figure 3

The effect of SIJ1777 on AKT and MAPK signaling pathways in melanoma cell lines harboring various BRAF mutation status (A) SK-MEL-2 (wt) (B) SK-MEL-28 (class I) (C) C8161 (class II) (D) WM3670, WM3629 (class III). Cells were treated with 0.01, 0.1, 1 μM of SIJ1777, and 1 μM of vemurafenib, PLX8394, GNF-7, and SIJ1227 for 2 h. Cell lysates were subjected to western blot analysis to estimate the phospho- or total- form of AKT, MEK, ERK levels, and GAPDH was used as the internal loading controls.