Figure 4.

The effect of SIJ1777 on apoptosis induction. (A) Western blot for pro-apoptotic marker level (cleaved PARP) in melanoma cell lines. GAPDH was used as the internal loading control. (B) Quantification graphs of western blot results by ImageJ (n = 3). (C) Apoptotic cell (annexin V-positive) population was measured by flow cytometry analysis against melanoma cell lines (n = 3). Cells were treated with indicated substances for 24 h. Statistical significances were determined using a one-way ANOVA analysis (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).