Figure 5.

The effect of SIJ1777 on migration and invasion of melanoma cells harboring BRAF wt or class I/II/II mutations. (A) Scratch assay results for assessing migration capability. After scratching each cell monolayer, indicated compounds at 0.01 μM concentration were incubated for 12 h. Migration ratio was analyzed using migrated area using ImageJ (n = 3). (B) Boyden chamber assay for assessing invasion capability using cell invasion kit (QCM ECMatrix Cell Invasion Assay, n = 3). Statistical significances were determined using a one-way ANOVA analysis (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).