Figure 3. Impact of α-MLT on glucose tolerance test, liver and abdominal fat weight, and hepatic steatosis in wild type mice on a high-fat diet.

Wild type male mice were fed with HFD for 12 weeks and then the mice were divided into two groups. One group of mice received α-MLT (1 mg/ml in drinking water) ad libitum for 3 weeks and the other group received drinking water alone ad libitum. At the end of 2 weeks, blood glucose was measured following 4 h fasting and then the intraperitoneal glucose tolerance test was performed (A,B). The diet and α-MLT administration continued another week; then the mice were sacrificed, and the weights of liver and abdominal fat pad were determined (C,D). Liver tissue was fixed and stained with Oil-O-red for neutral lipids (63×) (E). Data means ± S. E. M (N = 5 mice/group). * P < 0.05; *** P < 0.001.