Figure 5. Effect of α-MLT on body weight and its reversibility in ob/ob mice on a normal diet.

ob/ob male mice (3-month-old) were exposed to drinking water alone or to α-MLT (1 mg/ml) in drinking water. Body weight was measured throughout the experiment (A). After three weeks of treatment, mice were sacrificed, and the weights of the liver (B) and abdominal fat pad (C) were determined. Data are means ± S. E. M (N = 5 mice/group). * P < 0.05; *** P < 0.001. To assess the reversibility of α-MLT effect, the drug was withdrawn in the treatment group after 3-week treatment period, and the monitoring of the body weight continued for an additional 3 weeks (A). a, P < 0.001 compared with the control group; b, P < 0.01 and c, P < 0.001 compared with the body weight on the day of the drug withdrawal.