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. 2019 Jun 3;43(5):517–547. doi: 10.1093/femsre/fuz015

Figure 1.

Figure 1.

Overview approaches for the detection of fungal pathogens. Schematic representation of the different technologies used for the identification of fungal organisms. These techniques can be based on mass spectrometry (blue background), nucleic acid (red background) or antibody (orange background). Techniques based on more than one of these aspects are represented in the border of the respective divisions. ASP—allele-specific PCR; ARMS—amplification refractory mutation system; AS-Droplet-PCR—combination of ASP with droplet PCR; AS-qRT-PCR—combination of ASP with quantitative PCR; MLPA—multiplex ligation-dependent probe amplification; ddPCR—droplet digital PCR; FISH—fluorescent in situ hybridization; PNA-FISH—peptide nucleic acids-FISH; LNA—locked nucleic acids; RCA—rolling-circle amplification; LAMP—loop-mediated isothermal amplification; NASBA—nucleic acid sequence-based amplification; RFLP—restriction fragment length polymorphism; AFLP—amplified fragment length polymorphism; ELISA—enzyme-linked immunosorbent assay; MALDI-TOF MS—matrix-assisted laser desorption-time of flight mass spectrometry; PCR-ESI-MS—electrospray ionization mass spectrometry coupled with broad-spectrum PCR; SERRS—surface-enhanced resonance Raman spectroscopy; MCA—melting curve analysis; HRMA—high-resolution melting analysis.