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Manufactured human nasal mucosal epithelial cell sheets. (A) Human nasal mucosal epithelial cells cultured on temperature-responsive cell culture insert were harvested as contiguous transplantable cell sheets. Bars indicate 5 mm. (B) All manufactured cell sheets were confirmed to be stratified structure composed of epithelial cells. Bars indicate 25 μm. (C) Measurement of cytokeratin-positive cells in a cell sheet using flow cytometry.
