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Results of soft-agar colony-formation assay. To ascertain the tumorigenic potential of human mucosal nasal epithelial cells, the obtained human nasal mucosal epithelial cells were seeded in keratinocyte culture medium (KCM) containing 0.5% agar. Human fibroblasts and human nasal mucosal epithelial cells formed no colonies, whereas HeLa cells formed many after 15 days culture.
