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Assessment of gene-edited cattle.A, photographs of a gene-edited calve that carried the NRAMP1 insertion. B, the 5′ (left, 1824-bp) (top) and 3′ junction (right, 2261-bp) (bottom) PCR analyses confirming the site-specific targeting in the gene-edited cattle. The templates for PCR were genomic DNA samples that were extracted from the tissues of cattle. C, southern blot analyses of the genomic DNA extracted from gene-edited cattle. “WT” represents wild-type cattle. M, marker.
