Figure 10.

Analysis of algorithm parameters. All cells were repeatedly analyzed with the algorithm during development to characterize the effect of parameter choice on outcome. A, fraction of molecules remaining after grouping, normalized to the number of molecules with window size 1, as a function of temporal window size (frames, at 10 ms/frame), for Alexa Fluor 647 and ATTO 488. B, mean probability of chance association [p(chance association)] as a function of local neighborhood radius (nm). Each faded colored line is from a single cell. Black line shows the mean, gray lines indicate ±95% confidence interval.