Figure 5.

MEG3 localized in the nucleus of U2OS cells by epifluorescence and 2-color dSTORM.MEG3 was induced by treatment of U2OS-MEG3 cells for 20 h with 1 μg/ml doxycycline. Cells were stained for p53 with a secondary antibody conjugated to ATTO 488 (green) and either tiled oligonucleotides recognizing MEG3 (Quasar 670, magenta, A) or GAPDH (Quasar 570, magenta, B). From left to right: Merged image; RNA channel; p53 channel; dSTORM localization map; 30x inset of dSTORM localizations in the black box, with shaded circles indicating “molecules”. Scale bars are 10 μm, or 200 nm (right column). dSTORM, direct stochastic optical reconstruction microscopy.