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. 2021 Mar 12;296:100540. doi: 10.1016/j.jbc.2021.100540

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© 2021 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Nutlin-3a caused accumulation of p53 in the nucleus.MEG3 was induced by treatment of U2OS-MEG3 cells for 20 h with 1 μg/ml doxycycline and/or 10 μM nutlin-3a. Cells were stained for Mdm2 with a secondary antibody conjugated to ATTO 488 (green) and for p53 with a secondary antibody conjugated to Alexa Fluor 647 (magenta). From left to right: Merged image; RNA channel; p53 channel; dSTORM localization map; 30x inset of dSTORM localizations in the black box, with shaded circles indicating “molecules”. Scale bars are 10 μm or 200 nm (right column). dSTORM, direct stochastic optical reconstruction microscopy.