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Nanobodies reduce distance between fluorophores for bound pairs.A, nanobodies (pale green) can be used in the place of secondary antibodies, reducing the distance to ∼30 to 50 nm. B, nanobodies may also be used to bind directly to a target of interest, either native or via a small peptide fusion tag, reducing the distance to ∼10 to 30 nm. Antibody and nanobody graphics were created using NGL Viewer (56) from RCSB PDB 1IGT and 5IVO, respectively.
