Fig. 7.

Senescence and immuno-modulatory function of CD73⁺ kMSCs with aging. a, b Representative dot-plots (a) and percentage (b) of CD73⁺ kMSCs in Sca-1⁺ renal cells from young or aged kidneys after 7 days of culture (n = 8 per group). c, d Gene expression of Nt5e (CD73), Ccl2, Cx3cl1, Il10 (c) and Cdkn2a (p16) (d) by young or aged CD73⁺ kMSCs (n = 7–9 per group) and epithelial cells (n = 4 per group) relative to house-keeping gene. e, f Capillary western blot image (e) and quantification (f) of p18 protein expression relative to ß-actin in protein extract of young or aged CD73⁺ kMSCs (n = 5–8 per group) and epithelial cells (n = 4 per group). (g, h) IL-6 (g) and TNF-α (h) concentrations in co-culture supernatants of monocytes (mono) and young or aged CD73⁺ kMSCs stimulated or not for 24 h with LPS (ratio Sca-1⁺ : mono, 1:5) (n = 11–15 per conditions). *P < 0.05 **P < 0.01 ***P < 0.001 ns, not significant, with Mann–Whitney t test or Kruskal–Wallis with Dunn’s post-test