Figure 3. EVC2 down-regulation is likely to have reduced Hh signaling output in humans.
a. For each KEGG pathway, the ratio of Hu>Ch to Ch>Hu genes was tested. Asterisks mark pathways with FDR < 0.05 (binomial test). b. Chimpanzee to human expression ratio in hybrid iPSCs and CNCCs for skeleton-related genes that are differentially expressed in both cell types. EVC2 is the most down-regulated gene in humans compared to chimpanzees. c. EVC2 expression across all hybrid cell samples showing a ~4-fold mean decrease in human compared to chimpanzee in iPSCs and a ~6-fold mean decrease in CNCCs. Dashed line shows mean expression. Paired t-test P-values are shown.