Skip to main content
. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Nat Genet. 2021 Mar 1;53(4):511–520. doi: 10.1038/s41588-021-00798-y

Figure 5. NF-Y binding is affected by steric hindrance at the γ-globin promoters.

Figure 5.

(a) The sequence of γ-globin promoters. The eight sgRNAs used for dCas9 disruption experiment are shown below the sequence and color coded. The numbers (−208, etc) represent the distances between TSS and positions of the 17th nucleotide of each sgRNA. The position of LRF, BCL11A and NF-Y motifs are labeled in red. The flanking sequences of NF-Y motif is also involved in NF-Y binding.

(b) Right, RT-qPCR analysis of the percentage of γ-globin in BCL11A KO HUDEP-2 cells expressing dCas9 with different sgRNAs. Data is showed as mean (SD) of three technical replicates and a representative of two biological replicates. Left, NFYA CUT&RUN in these cells. The cartoon indicates the deduced protein binding at the promoters.