Fig. 7.

PGD₂ suppressed EMT in cultured kidney cells. a Effects of lipid metabolites which were significantly different between Alox15^+/+ and Alox15^−/− mice under 5/6 Nx condition on the EMT of NRK-52E cells. These lipid metabolites were administered at 8 μM each to NRK-52E cells with TGF-β1 (5 ng/mL). PGD₂ significantly suppressed Col1a1 and αSMA (Acta2) expression. None of the other fatty acid metabolites inhibited or promoted Col1a1 and αSMA. b Effects of PGD₂ on the EMT of NRK-52E cells. PGD₂ significantly suppressed Col1a1 and Acta2 expression induced with TGF-β1 (5 ng/mL) in NRK-52E cells dose-dependently. c Effects of PGD₂ on the EMT of HK-2 cells. PGD₂ also suppressed Col1a1 and Acta2 expression induced with TGF-β1 (5 ng/mL) in HK-2 cells dose-dependently. n = 3 for each group. Values are mean ± SEM. One-way analysis of variance was followed by Tukey’s multiple comparisons test, *P < 0.05