Fig. 8.

15-PGDH, a major PGD₂-metabolizing enzyme, was reduced in Alox15^−/− CKD kidneys. a Relative mRNA levels of each of the two PGDS synthase isoforms in the kidney tissue of sham or CKD model mice. In WT mice, both L-PGDS and H-PGDS levels were significantly increased under 5/6 Nx conditions when compared with those under sham conditions (both P < 0.0001). Conversely, the increase in L-PGDS and H-PGDS under 5/6 Nx conditions was significantly inhibited in Alox15^−/− mice (P = 0.0004, < 0.0001, respectively), and their mRNA levels did not differ from those of sham WT mice. The number of samples is shown at the bottom of the bar graph. Values are mean ± SEM. One-way analysis of variance was followed by Tukey’s multiple comparisons test, *P < 0.05. b Relative mRNA levels of PGD₂-related enzymes in the kidneys of CKD model mice. The mRNA level of 15-PGDH was significantly reduced in CKD kidneys of Alox15^−/− mice when compared with those of WT mice (P = 0.0325). The mRNA levels of COX-1, COX-2 and AKR1C18 were not significantly changed between WT mice and Alox15^−/− mice under 5/6 Nx conditions. The number of samples is shown at the bottom of the bar graph. Values are mean ± SEM. Unpaired Student’s t test, *P < 0.05