Table 1.
List of lipid metabolites which were significantly different between Alox15+/+ and Alox15−/− mice under 5/6 Nx condition
| Sample name | Sham WT (n = 6) | Sham KO (n = 6) | Nx WT (n = 4) | Nx KO (n = 4) |
P value (Nx WT vs Nx KO) |
Increased or decreased in KO compared to WT | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Ave | SE | Ave | SE | Ave | SE | Ave | SE | |||
| PGD2 | 10.0 | 1.5 | 16.5 | 5.9 | 53.4 | 14.5 | 163.1 | 45.1 | 0.0093 | Increased |
| 15-HEPE | 78.8 | 8.7 | 99.4 | 8.1 | 219.2 | 26.7 | 89.5 | 25.9 | 0.0006 | Decreased |
| 18-HEPE | 136.9 | 13.3 | 215.7 | 17.6 | 260.8 | 55.7 | 118.3 | 21.7 | 0.0186 | Decreased |
| 10-HDoHE | 128.8 | 16.5 | 256.9 | 30.7 | 288.1 | 54.7 | 126.7 | 25.4 | 0.0246 | Decreased |
| 11-HDoHE | 245.9 | 43.2 | 488.0 | 74.5 | 678.4 | 146.6 | 305.5 | 57.8 | 0.0443 | Decreased |
| 13-HDoHE | 140.8 | 17.4 | 255.2 | 26.8 | 388.1 | 104.6 | 162.8 | 31.2 | 0.0341 | Decreased |
| 14-HDoHE | 289.5 | 25.1 | 368.6 | 26.2 | 673.2 | 128.3 | 328.0 | 57.6 | 0.0092 | Decreased |
| 16-HDoHE | 232.1 | 25.1 | 369.1 | 38.3 | 471.7 | 106.7 | 227.0 | 53.4 | 0.0497 | Decreased |
| 17-HDoHE | 468.5 | 82.0 | 513.1 | 59.0 | 1253.0 | 218.2 | 350.6 | 88.7 | 0.0005 | Decreased |
| DGLA | 9794.6 | 349.6 | 8797.8 | 791.8 | 22,167.8 | 3136.0 | 13,040.0 | 2866.7 | 0.0195 | Decreased |
By mediator lipidomics, the above fatty acid metabolites were detected in the kidney tissue (30 mg). The P values in the table were obtained by comparing Alox15+/+ and Alox15−/− mice under 5/6 Nx condition. The number of samples is as follows: sham (WT), n = 6, sham (KO), n = 6, Nx (WT), n = 4, Nx (KO), n = 4. One-way analysis of variance was followed by Tukey’s multiple comparisons test