Fig. 2.

Flotillins are recruited to the plasma membrane upon LecA stimulation. a Fluorescence co-localization studies of flotillin-1 (red) and LecA (green) after 60 min of lectin stimulation. Nuclei were counterstained by DAPI. Framed areas were magnified. White arrows point at co-localization events at the plasma membrane, asterisks at perinuclear co-localization. Scale bar: 10 μm. b Mander’s co-localization coefficient quantified between the fluorescence signals of flotillin-1 and LecA were statistically compared to time point 0. Bars display mean values of at least three biological replicates, error bars represent SD, *p < 0.05, ***p < 0.001 (one-way ANOVA and Dunnett’s multiple comparisons test). c Pull-down of LecA-biotin resulted in a time-dependent enrichment of flotillin-1. d LecA was co-immunoprecipitated with flotillin-1 after 30 min of LecA treatment, the binding and precipitation was inhibited by PPMP treatment