Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 8;78(7):3637–3656. doi: 10.1007/s00018-021-03766-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021, corrected publication 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — The LecA plasma membrane domain includes CD59 proteins. a Fluorescence co-localization studies of CD59 (red) and LecA (green) after 60 min of lectin stimulation. Nuclei were counterstained by DAPI. Framed areas were magnified. White arrows point at co-localization events at the plasma membrane, asterisks at perinuclear co-localization. Scale bar: 10 μm. b Signal overlay of LecA and CD59 is displayed by Mander’s co-localization coefficient and statistically compared to time point 0. Bars display mean values of at least four biological replicates, error bars represent SD, ****p < 0.0001 (one-way ANOVA and Dunnett’s multiple comparison tests). c CD59 was validated as component of the LecA-binding domain by pull-down of LecA-biotin. d Immunoprecipitation of CD59 co-precipitated LecA after 30 min of stimulation. PPMP treatment to deplete cells in glucosylceramide-based GSLs, such as Gb3, largely inhibited the precipitation of LecA