Fig. 4.

Src family kinases and Rac1 are activated upon LecA stimulation. a Src family kinases are phosphorylated upon LecA treatment of H1299 cells as demonstrated by immunoblot analysis. b Src activation was quantified by the ratio between phospho-Src (Tyr⁴¹⁶) and Src and normalized to control levels. Bars display mean values of at least four biological replicates, error bars represent SD, *p < 0.05 (one-way ANOVA and Dunnett’s multiple comparisons test). c The Src family kinase was pulled-down together with LecA after 15 and 30 min of treatment. d G-LISA of Rac1 demonstrated a non-significant activation of the small G protein Rac1 upon LecA stimulation. Values were normalized to control levels. Bars display mean values of three biological replicates, error bars represent SD