Fig. 7.

Flotillins and CD59 promote cellular invasion of P. aeruginosa in conjunction with LecA. a CRISPR-Cas9 knockout of FLOT1 and siRNA transfection of FLOT2 depleted H1299 cells almost completely of flotillins. Note, that protein levels of flotillins are interdependent. b CD59 was efficiently knocked-down by siRNA transfection of WT H1299 cells. c Invasion assay of WT, flotillin- and CD59-depleted H1299 cells using WT PAO1 and ΔLecA mutant PAO1 strains demonstrated the impact of flotillins and CD59 on PAO1 invasion. Invasion ratio was normalized to WT cells and WT PAO1. Bars display mean values of at least three biological replicates, error bars represent SD, **p < 0.01. ***p < 0.001 (one-way ANOVA and Dunnett’s multiple comparisons test)