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. 2021 Apr 7;13:3041–3053. doi: 10.2147/CMAR.S290088

Figure 2.

Figure 2

Influence of circ-OGDH inhibition on proliferation, metastasis, invasion, and glutamine metabolism of ESCC cells. (A) QRT-PCR revealed the expression of circ-OGDH in TE-1 and Eca-109 cells transfected with si-circ-OGDH#1, si-circ-OGDH#2, or si-NC. *P < 0.05 and **P < 0.01 vs si-NC. (B–L) TE-1 and Eca-109 cells were transfected with si-circ-OGDH#1 (si-circ-OGDH) or si-NC. (B–D) The viability, colony formation, and apoptosis of TE-1 and Eca-109 cells were determined by MTT assay, colony formation assay, or flow cytometry assay. **P < 0.01 vs si-NC. (E–H) The migration, invasion, and cell cycle progression of TE-1 and Eca-109 cells were analyzed by transwell assay or flow cytometry assay. **P < 0.01 vs si-NC. (I–K) Glutamine consumption, α-KG production, and ATP content in TE-1 and Eca-109 cells were assessed using matching kits. **P < 0.01 vs si-NC. (L) Western blotting presented the level of GLS1 protein in TE-1 and Eca-109 cells. **P < 0.01 vs si-NC.