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. 2021 Apr 7;13:3041–3053. doi: 10.2147/CMAR.S290088

Figure 3.

Figure 3

Circ-OGDH served as a sponge for miR-615-5p in ESCC cells. (A) Circular RNA interactome prediction exhibited that circ-OGDH might be a sponge of miR-615-5p. (B) Schematic illustration of a luciferase reporter containing wt-circ-OGDH or mut-circ-OGDH. (C) Dual-luciferase reporter assay was carried out to analyze the luciferase activity of the luciferase reporter containing wt-circ-OGDH or mut-circ-OGDH. **P < 0.01 vs miR-NC. (D) QRT-PCR revealed the enrichment of miR-615-5p and circ-OGDH in RNA complexes in the Ago2 and IgG groups. ***P < 0.001 vs IgG. (E and F) QRT-PCR analyzed the expression of miR-615-5p in ESCC tissues and cells. **P < 0.01 vs para-carcinoma tissues or HEEC cells. (G) QRT-PCR verified the overexpression efficiency of circ-OGDH in TE-1 and Eca-109 cells. ***P < 0.001 vs Vector. (H) QRT-PCR analysis of the influence of circ-OGDH overexpression or inhibition on miR-615-5p expression in TE-1 and Eca-109 cells. **P < 0.01 vs Vector or si-NC.