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. 2021 Mar 29;12:649492. doi: 10.3389/fphys.2021.649492

FIGURE 3.

FIGURE 3

Brca1 and Brca2 in neural crest cells are critical for palatogenesis in mice. (A) Brca1 and Brca2 were expressed in mouse palate tissues. The corresponding sense probe was used as a negative control. PS, palatal shelf. (B) Neural crest-specific disruption of Brca1 and Brca2 induced cleft palate (asterisks). (C) H&E staining of sections from control and Brca1 cKO embryos from E12.5 to E14.5. Asterisk shows cleft palate. PS, palatal shelf; T, tongue. (D) The quantification analysis of the palatal shelves area from control and Brca1 cKO embryos at E12.5 and E13.5. PS, palatal shelf. (E) H&E staining of sections from control and Brca2 cKO embryos from E12.5 to E14.5. Asterisk shows cleft palate. (F) The quantification analysis of the palatal shelves area from control and Brca2 cKO embryos at E12.5 and E13.5. PS, palatal shelf. Data in panels (D,F) are represented as mean ± SD, n = 3 in each group. *p < 0.05; **p < 0.01.