Skip to main content
. 2021 Mar 29;12:645741. doi: 10.3389/fimmu.2021.645741

Figure 5.

Figure 5

PM induces IL-1β secretion and acute neutrophilia via Tγδ17 cells. (A–C) B6 mice were intranasally administered with 250 μg of PM or PBS and single cell suspensions of lung tissue were analyzed at 4 hours after PM exposure. (A, B) Representative dot plots show alveolar macrophages (A) and neutrophils (B). Bar graphs show statistical analysis of absolute number of pro-IL-1β-producing cells. (C) Pie charts show mean frequencies (proportional to angle) and numbers (proportional to area) of indicated cells among total pro-IL-1β-producing cells. (D, E) B6 mice were intranasally administered with 250 μg of PM and analyzed at indicated time points. (D) Representative dot plots show alveolar macrophages (AM), neutrophils (NEU) and eosinophils (EO) in broncho-alveolar lavage fluid (BALF) harvested at 12 hours after PM administration. (E) Graphs show the numbers of alveolar macrophages and neutrophils at indicated time periods in BALF (N = 2 ~ 4). (F–K) B6 mice were intranasally administered with 250 μg of PM and mononuclear cells of lung tissue were analyzed at 24 hours after PM exposure. Representative contour plots show IL-17A+ or IL-17F+ pulmonary Tγδ17 (F) MAIT17 (G) NKT17 (H) Th17 (I) and ILC3 cells (J). Bar graph shows statistical analysis of frequencies of IL-17A-producing cells. (K) Pie charts show mean frequencies (proportional to angle) and numbers (proportional to area) of indicated cells among total IL-17A-producing pulmonary CD45+ cells. Numbers indicate frequencies of cells in adjacent gates or frequencies in each area (C, K). Each dot represents an individual mouse and error bars indicate ± SD. Unpaired two-tailed t-test was used. N.S, not significant, *P <0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. AM, alveolar macrophage; IM, interstitial macrophage; NEU, neutrophils; EO, eosinophil.