Figure 3.

Nox4 protein expression in human TM cells. Serum-starved confluent cultures of primary or transformed TM cells were treated in the absence (A) or presence (B, C) of vehicle (0.1% DMSO) or TGF-β2 (5 ng/mL for 24 hours), as indicated. A Immunoblots of Nox4 proteins in lysates from quiescent primary or transformed (GTM3) TM cells, as indicated. B and C Nox4 proteins expressed in lysates from primary B or transformed C TM cells treated without (vehicle) or with TGF-β2, as indicated. GAPDH, glyceraldehyde phosphate dehydrogenase protein loading controls. (Bottom panels) Densitometric analyses of respective Nox4 protein bands. Data shown are the means ± SD (N = 3 replicate wells per condition) of GAPDH-normalized Nox4 protein bands from a single experiment representative of two separate experiments. ns, not significant; *P < 0.05; ***P < 0.0001, Student's t-test.