Figure 7.

A dual inhibitor of Nox1-Nox4 enzyme activity attenuates TGF-β2 mediated collagen protein expression. Serum-starved confluent cultures of primary TM cells were pretreated (2 hours) with SB-431542 (10 µM) or GKT137831 (20 µM) and incubated with vehicle (0.1% DMSO) or TGF-β2 (5 ng/mL) for an additional 22 hours, as indicated. (A) Representative confocal immunofluorescent images of collagens I (green) or collagens IV (red) proteins expressed in treated cells. Blue, DAPI-stained nuclei. Scale Bar, 100 µm. (B, C) Quantitative comparison of B collagen I or C collagen IV immunofluorescence. Data shown are the means ± SD (N = 4–8 separate wells per condition, 2 fields per well, 15 cells per field). **P < 0.001; ***P < 0.0001, 1-way ANOVA with Tukey's post hoc analysis.