Figure 2.

Function analysis for Gtf2i and HTRA1.A, HTRA1 in/del and normal with or without Gtf2i β/δ vectors were transfected in HEK-293, 661W and COS-7 cells, respectively, following ELISA. Presence of the in/del significantly enhanced HTRA1 secretion and Gtf2i β/δ promoted enhanced efficiency with indel-HTRA1 but not the normal-HTRA1 in all cell lines. B, secretion of HTRA1 in human iPSCs derived from AMD patients. iPSCs were derived from individuals with normal versus in/del transcription regulators, followed by ELISA. Transfection of 661W cells with the Gtf2i β or δ expression vectors significantly enhanced in/del-Luciferase transcription comparing with normal. Representative WB (C) and immunocytochemistry (D) results are shown. Throughout, the results are expressed as the mean ± SEM. The p value was obtained by Student's t test.